"The borders may close soon because of the new variant," said Eugene Ho, a 31-year-old banker leaving Singapore for the first time in nearly two years. "I am actually very worried about getting stuck."
Travellers must test negative for COVID-19 before departure, and Malaysia also requires travellers to get an on-arrival test, a move Singapore followed on Sunday over concerns about the Omicron variant.
Malaysian Prime Minister Ismail Sabri Yaakob was welcomed by Singaporean Prime Minister Lee Hsien Loong at one of the land border crossings on Monday, his first official visit as premier to Singapore.
Siva Kumar, a 41-year-old engineer in the semiconductor industry, had been inundated with calls from his two teenage sons waiting eagerly for his return.
"All morning they keep calling, 'Where are you now Have you taken the bus yet'," Kumar said. "(I want to) hug them, kiss them. I've really missed them."
As many as 300,000 Malaysians commuted daily to Singapore before the pandemic.
The sudden closing of the border in March 2020 left tens of thousands stranded on both sides, separated from families and fearing for their jobs.
Under the new arrangement, up to 1,440 travellers from each side can cross the land border each day without quarantine, if they hold citizenship, permanent residency or long-term visas in the destination country, according to guidelines published by the Singapore government.
A vaccinated air travel lane between the two countries also started on Monday.
Singapore has vaccinated 85% of its entire population, while about 80% of Malaysia's population has been inoculated.
Singapore, with an aging population of 5.5 million, relies heavily on Malaysians living in the southern state of Johor to staff businesses ranging from restaurants to semiconductor manufacturing.
Singapore reported 747 locally acquired COVID-19 cases on Sunday, the lowest tally since mid-September. Malaysia reported 4,239 cases on Sunday, the smallest number since early November.
(Reporting by Joe Brock and Chen Lin; Editing by John Geddie, Ed Davies, Karishma Singh and Gerry Doyle) ((Lin.Chen@tr.com; Twitter @chenlin_sg;))