- Alzheimer’s disease, an irreversible and progressive brain disorder, is the most common causeof dementia.
- A hallmark of Alzheimer’s disease is the aggregation of the amyloid-β (Aβ) protein in a process that is toxic to the brain’s neurons. As neurons die, this slowly destroys memory and cognitive function.
- NYUAD researchers have developed small proteins called cell-penetrating peptides (CPPs) that prevent the aggregation of the Aβ protein.
- The paper Designed cell-penetrating peptide inhibitors of amyloid-beta aggregation and cytotoxicity has been published in Cell Reports Physical Science.
Abu Dhabi: A team of researchers, led by NYU Abu Dhabi Assistant Professor of Biology Mazin Magzoub, has developed small proteins called cell-penetrating peptides (CPPs) that prevent the aggregation of the amyloid-β (Aβ) protein associated with Alzheimer’s disease.
There is a growing interest in the use of proteins to develop treatments because they are biocompatible, biodegradable, and can selectively bind to specific targets, which reduces the potential for toxicity. Other pharmaceutically-desirable properties of proteins include greater chemical diversity than other biological molecule classes, and ease and low cost of production. However, a significant obstacle to the successful application of most proteins as therapeutics is their poor delivery to target organs and cells.
In a new study, the Magzoub lab in collaboration with Professor and NYU President Andrew Hamilton’s lab at NYU New York and Professor Astrid Gräslund’s lab at Stockholm University, report that they have engineered CPPs that effectively prevent the aggregation of Aβ. CPPs are a special class of proteins that have the ability to reach all organs, including the brain, and to efficiently enter cells within these organs. Thus, the designed CPPs combine the attractive properties of proteins with potent therapeutic effects and highly efficient delivery to target cells.
A broad range of neurodegenerative diseases, including Huntington’s, Parkinson’s, and Alzheimer’s, are caused by the misfolding of disease-specific proteins. Once misfolded, these proteins build up in a process that damages neurons. Previously, the team showed that a naturally-derived CPP was able to protect against abnormal and harmful forms of the protein associated with prion diseases, a class of neurodegenerative disorders that include mad cow disease in cattle and Creutzfeldt-Jakob disease (CJD) in humans. Here, Magzoub and his collaborators extended this approach and specifically applied it to Alzheimer’s disease-causing Aβ malformation.
In the paper titled Designed cell-penetrating peptide inhibitors of amyloid-beta aggregation and cytotoxicity, the researchers present the process of designing the Aβ inhibitor CPPs. Using a range of techniques, they then extensively characterized the interactions of the designed CPPs with Aβ. Specifically, they used established aggregation and cell viability assays to determine the effects of the designed CPPs on the aggregation and associated neurotoxicity of Aβ. Simultaneously, they probed the cellular uptake and intracellular distribution of Aβ in the presence of the CPPs with confocal fluorescence microscopy. Finally, they used a combination of experiments and computer simulations to shed light on the mechanism of binding of the CPPs to Aβ. The results show that the designed CPPs stabilize the Aβ protein in a non-aggregated, non-abnormal state, and inhibit Aβ induced neurotoxicity.
The CPPs designed in this study effectively target Aβ outside and inside neurons, thereby protecting them against the damage caused by Aβ aggregation. “The designed CPPs represent a novel potential treatment strategy for Alzheimer’s disease,” said Magzoub. “These findings also reveal a general underlying principle for inhibition of pathogenic protein aggregation that will facilitate the design of even more potent CPP-based therapeutics for various neurodegenerative diseases.”
About NYU Abu Dhabi
NYU Abu Dhabi is the first comprehensive liberal arts and science campus in the Middle East to be operated abroad by a major American research university. NYU Abu Dhabi has integrated a highly-selective liberal arts, engineering and science curriculum with a world center for advanced research and scholarship enabling its students to succeed in an increasingly interdependent world and advance cooperation and progress on humanity’s shared challenges. NYU Abu Dhabi’s high-achieving students have come from 115 nations and speak over 115 languages. Together, NYU's campuses in New York, Abu Dhabi, and Shanghai form the backbone of a unique global university, giving faculty and students opportunities to experience varied learning environments and immersion in other cultures at one or more of the numerous study-abroad sites NYU maintains on six continents.© Press Release 2020
Disclaimer: The contents of this press release was provided from an external third party provider. This website is not responsible for, and does not control, such external content. This content is provided on an “as is” and “as available” basis and has not been edited in any way. Neither this website nor our affiliates guarantee the accuracy of or endorse the views or opinions expressed in this press release.
The press release is provided for informational purposes only. The content does not provide tax, legal or investment advice or opinion regarding the suitability, value or profitability of any particular security, portfolio or investment strategy. Neither this website nor our affiliates shall be liable for any errors or inaccuracies in the content, or for any actions taken by you in reliance thereon. You expressly agree that your use of the information within this article is at your sole risk.
To the fullest extent permitted by applicable law, this website, its parent company, its subsidiaries, its affiliates and the respective shareholders, directors, officers, employees, agents, advertisers, content providers and licensors will not be liable (jointly or severally) to you for any direct, indirect, consequential, special, incidental, punitive or exemplary damages, including without limitation, lost profits, lost savings and lost revenues, whether in negligence, tort, contract or any other theory of liability, even if the parties have been advised of the possibility or could have foreseen any such damages.